Competitive Protein Binding Analysis for Thyroxine Using Sephadex Column (Tetralute)
نویسندگان
چکیده
منابع مشابه
Evaluation of new kits for the assessment in vitro of thyroid function by determination of serum total thyroxine, free TBG capacity, and free thyroxine index using Sephadex G-25 and '25I-labelled triiodothyronine and thyroxine
SYNOPSIS A trial was carried out on 134 patients of new kits (Ames Co) using columns of Sephadex G-25 for the determination of serum total thyroxine (Tetralute test) and for the indirect estimation of serum free thyroxine-binding globulin capacity (Trilute test). Both new methods were quicker and easier than the reference resin methods and of similar precision. The two measurements when combine...
متن کاملtotal thyroxine, free TBG capacity, and free thyroxine index using Sephadex G-25 and '25I-labelled triiodothyronine and thyroxine
SYNOPSIS A trial was carried out on 134 patients of new kits (Ames Co) using columns of Sephadex G-25 for the determination of serum total thyroxine (Tetralute test) and for the indirect estimation of serum free thyroxine-binding globulin capacity (Trilute test). Both new methods were quicker and easier than the reference resin methods and of similar precision. The two measurements when combine...
متن کاملAn automated competitive binding procedure for measuring thyroxine in serum.
A competitive binding assay for serum thyroxine has been automated, with the use of small, reusable Sephadex columns to separate thyroxine from endogenous thyroid-binding globulin and later to separate the bound and free thyroxine. Sixty samples an hour are run through columns, which are held in an aluminum turntable rotated by a fraction collector motor. Reagents and samples are fed to the col...
متن کاملRadioimmunoassay for normalized thyroxine with use of Sephadex columns.
We describe a reproducible radioimmunoassay, with use of Sephadex columns, for measuring normalized thyroxine. In comparison with a competitive protein-binding procedure, the present method is more specific because antibody rather than thyroxine-binding globulin is responsible for the competition between endogeneous and tracer thyroxine. In barbital buffer, various concentrations of thyroxine b...
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ژورنال
عنوان ژورنال: RADIOISOTOPES
سال: 1975
ISSN: 1884-4111,0033-8303
DOI: 10.3769/radioisotopes.24.11_763